Coding

Part:BBa_K5257011:Design

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


merR tn501


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized codons for synthesis and addition of TAA stop codon.



Source

Ralston, D. M., & O'Halloran, T. V. (1990). Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex. Proceedings of the National Academy of Sciences, 87(10), 3846-3850. https://doi.org/10.1073/pnas.87.10.3846

Wang, D., Huang, S., Liu, P., Liu, X., He, Y., Chen, W., & Chen, H. (2016). Structural analysis of the Hg (II)-regulatory protein Tn501 MerR from Pseudomonas aeruginosa. Scientific Reports, 6(1), 33391. https://doi.org/10.1038/srep33391

Wang, D., Zheng, Y., Wei, L., Wei, N., Fan, X., Huang, S., & Xiao, Q. (2020). A signal-amplified whole-cell biosensor for sensitive detection of Hg2+ based on Hg2+-enhanced reporter module. Journal of Environmental Sciences, 96, 93-98. https://doi.org/10.1016/j.jes.2020.03.020

References