Coding

Part:BBa_K5257010

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-29)


merR m4-1 variant

The merR m4-1 variant is a mutant of merR tn21 in D68G/L88M/H91R/V109E/S111T that allows it to have higher affinity to DNA and to be sensitive to organic mercury species such as methylmercury, ethylmercury and phenylmercury nitrate.It has a higher affinity for inorganic mercury.

The gene sequence was obtained from Zhu et al. (2023).

References

Zhu, K., Chen, D., Cai, Y., Zhang, T., Ma, J., Bao, L., & Chen, S. (2023). Engineering the Ultrasensitive Visual Whole-Cell Biosensors by Evolved MerR and 5′ UTR for Detection of Ultratrace Mercury. Environmental Science & Technology, 57(44), 16964-16973. https://doi.org/10.1016/j.jhazmat.2024.135578


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None