Part:BBa_K5257006

gomerB T6B2 variant

Enzyme goMerB T6-B2 (alkyl mercury lyase) is a mutant of the MerB enzyme from Gordonia otitis (goMerB) with increased efficiency of catalytic biological digestion of organic mercury.

The enzyme is 22 kDa in size and with a catalytic site at cys95, Asp98 and cys160.

The gene sequence was obtained from Guo et al. (2024).

Characterized and developed by Guo et al. (2024).

References

Guo, M., Chen, X., Chen, S., Su, H., Liu, H., Xie, G., & Sun, B. (2024). Replacing manual operation with bio-automation: A high-throughput evolution strategy to construct an integrated whole-cell biosensor for the simultaneous detection of methylmercury and mercury ions without manual sample digestion. Journal of Hazardous Materials, 465, 133492. https://doi.org/10.1016/j.jhazmat.2024.133492

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 381
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]