Reporter
Part:BBa_K5257004:Design
Designed by: Oskar Samuel Zeballos Huarachi Group: iGEM24_Bolivia (2024-09-28)
sfGFP mChartreuse variant
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 674
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 674
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 674
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 674
Illegal AgeI site found at 25
Illegal AgeI site found at 148 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
References Bao, L., Menon, P. N. K., Liljeruhm, J., & Forster, A. C. (2020). Overcoming chromoprotein limitations by engineering a red fluorescent protein. Analytical biochemistry, 611, 113936. https://doi.org/10.1016/j.ab.2020.113936