Reporter

Part:BBa_K5257004

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-28)


sfGFP mChartreuse variant

mChartreuse fluorescent protein is mutant of sfGFP in N39I/R80Q/I128S/D129G/F145Y/N149K/V206K with a low molecular weight (26.7 kDa), its maturation time is faster than sfGFP. It is a fluorescent protein with an Excitation Maximum of 487 nm and an Emission Maximum of 510 nm.

The gene sequence was obtained from Addgene https://www.addgene.org/219397/ The amino acid sequence is also available at https://www.fpbase.org/protein/mchartreuse/ Characterization was performed by Fraikin et al. (2024).

References Fraikin, N., Couturier, A., & Lesterlin, C. (2024). A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging. bioRxiv, 2024-03. https://doi.org/10.1101/2024.03.28.587235


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 674
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 674
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 674
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 674
    Illegal AgeI site found at 25
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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