Part:BBa_K5257004
sfGFP mChartreuse variant
mChartreuse fluorescent protein is mutant of sfGFP in N39I/R80Q/I128S/D129G/F145Y/N149K/V206K with a low molecular weight (26.7 kDa), its maturation time is faster than sfGFP. It is a fluorescent protein with an Excitation Maximum of 487 nm and an Emission Maximum of 510 nm.
The gene sequence was obtained from Addgene https://www.addgene.org/219397/ The amino acid sequence is also available at https://www.fpbase.org/protein/mchartreuse/ Characterization was performed by Fraikin et al. (2024).
References Fraikin, N., Couturier, A., & Lesterlin, C. (2024). A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging. bioRxiv, 2024-03. https://doi.org/10.1101/2024.03.28.587235
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 674
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 674
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 674
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 674
Illegal AgeI site found at 25
Illegal AgeI site found at 148 - 1000COMPATIBLE WITH RFC[1000]
None |