Reporter

Part:BBa_K5257000:Design

Designed by: Oskar Samuel Zeballos Huarachi   Group: iGEM24_Bolivia   (2024-09-26)


mRFP1 magenta variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The gene sequence was obtained from Addgene https://www.addgene.org/160446/ The amino acid sequence is also available at https://www.fpbase.org/protein/mrfp1_magenta/ Characterization was performed by Bao et al. 2020. Bao, L., Menon, P. N. K., Liljeruhm, J., & Forster, A. C. (2020). Overcoming chromoprotein limitations by engineering a red fluorescent protein. Analytical biochemistry, 611, 113936.


References