Coding
BisdAB
Part:BBa_K525515:Design
Designed by: Anna Drong Group: iGEM11_Bielefeld-Germany (2011-09-10)
Fusion protein of BisdA and BisdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 603
Illegal BamHI site found at 1341 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 1594 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Fusion protein of BisdA and BisdB in Freiburg BioBrick assembly standard (RFC 25).
Source
- bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
References
Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].