Composite

Part:BBa_K5253009

Designed by: Anna Kristensen   Group: iGEM24_SDU-Denmark   (2024-08-27)


Allows for the expression of the RFP gene under the control of the CD47 promoter, with transcription

This composite part consists of three key genetic elements:

CD47 Promoter: A regulatory DNA sequence that initiates the transcription of downstream genes, specifically in cells expressing CD47, which is involved in immune evasion.

RFP Gene: A gene that encodes the Red Fluorescent Protein (RFP), a marker protein that emits red fluorescence when expressed, commonly used in molecular biology to visualize and track gene expression.

SV40 Terminator: A DNA sequence derived from Simian Virus 40 (SV40) that signals the end of transcription, ensuring the proper termination and polyadenylation of the mRNA transcript.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1346
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1346
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1346
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1346
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None