Composite

Part:BBa_K5253008

Designed by: Anna Kristensen   Group: iGEM24_SDU-Denmark   (2024-08-27)


Allow for the controlled expression of the BCL-2 gene under the regulation of the BRCA-1 promoter, w

This composite part includes three important genetic elements:

BRCA-1 Promoter: A regulatory DNA sequence that controls the expression of downstream genes, typically active in cells where BRCA-1 plays a role. BRCA-1 is involved in DNA repair and maintaining genomic stability.

BCL-2 Gene: An anti-apoptotic gene that encodes the BCL-2 protein, which inhibits cell death by preventing the release of pro-apoptotic factors from mitochondria, promoting cell survival.

SV40 Terminator: A sequence from Simian Virus 40 (SV40) that ensures proper transcription termination and polyadenylation, allowing for the stable production of mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 834
    Illegal SpeI site found at 55
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 834
    Illegal SpeI site found at 55
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 834
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 834
    Illegal SpeI site found at 55
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 834
    Illegal SpeI site found at 55
    Illegal AgeI site found at 1025
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 460
    Illegal BsaI.rc site found at 643
    Illegal BsaI.rc site found at 1194


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Categories
Parameters
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