Composite

Part:BBa_K5253007

Designed by: Anna Kristensen   Group: iGEM24_SDU-Denmark   (2024-08-27)


Drive the expression of the BAX gene under the control of the CD47 promoter, with transcription prop

This composite part consists of three key genetic elements:

CD47 Promoter: A regulatory DNA sequence that drives the expression of downstream genes, specifically by promoting transcription. CD47 is involved in immune evasion by preventing phagocytosis.

BAX Gene: A pro-apoptotic gene that encodes the BAX protein, which promotes cell death by facilitating the release of cytochrome c from mitochondria, leading to apoptosis.

SV40 Terminator: A DNA sequence derived from the Simian Virus 40 (SV40) that signals the end of transcription, ensuring proper termination and polyadenylation of mRNA transcripts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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