Device

Part:BBa_K5250031

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-02)


Prha, CcdB + Prha, CcdA

This is a l Level 2 testing plasmid assemble with Golden Gate. It is part of the CcdB/CcdA-based kill switch in our construct and expresses both the CcdB toxin and CcdA antitoxin after a rhamnose promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 483
    Illegal BamHI site found at 919
    Illegal BamHI site found at 1799
    Illegal BamHI site found at 2679
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1660
    Illegal AgeI site found at 1772
    Illegal AgeI site found at 2540
    Illegal AgeI site found at 2652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 350


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Categories
Parameters
None