Device

Part:BBa_K5250026

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter, T7-like RNAP + pT7-like, T7-like RNAP + pT7-like, GFP


The first transcription unit expresses constitutively the T7-like RNAP (BBa_K5250002) originating from the Pseudomonas phage Pf-10. This then activates two T7-like promoters (BBa_K5250003) in the next two transcription units. One of them expresses another T7-like RNAP, while the other one - GFP.

This is a Level 2 plasmid constructed with Golden Gate assembly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2715
    Illegal BamHI site found at 3510
    Illegal BamHI site found at 6261
    Illegal BamHI site found at 7141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2898
    Illegal AgeI site found at 3359
    Illegal AgeI site found at 7002
    Illegal AgeI site found at 7114
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1220
    Illegal BsaI site found at 2090
    Illegal BsaI site found at 3702
    Illegal BsaI site found at 4766
    Illegal BsaI site found at 5636
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 600
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 2538
    Illegal BsaI.rc site found at 3654
    Illegal BsaI.rc site found at 4146
    Illegal BsaI.rc site found at 5074
    Illegal SapI site found at 2519
    Illegal SapI site found at 6065
    Illegal SapI.rc site found at 1714
    Illegal SapI.rc site found at 5260


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Categories
Parameters
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