Device

Part:BBa_K5250025

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter, RNAP + pT7-like, RFP + pT7-like, GFP

This is a Level 2 plasmid assembled with Golden Gate. T7-like RNAP (BBa_K5250002) is constitutively expressed, which activates two T7-like promoters (BBa_K5250003), one of them is expressing GFP, the other - RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2715
    Illegal BamHI site found at 3510
    Illegal BamHI site found at 4287
    Illegal BamHI site found at 5167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2898
    Illegal AgeI site found at 3359
    Illegal AgeI site found at 4148
    Illegal AgeI site found at 4260
    Illegal AgeI site found at 5028
    Illegal AgeI site found at 5140
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1220
    Illegal BsaI site found at 2090
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 600
    Illegal BsaI.rc site found at 1528
    Illegal BsaI.rc site found at 2538
    Illegal SapI site found at 2519
    Illegal SapI.rc site found at 1714


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