Composite

Part:BBa_K5250023:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


pT7-like + T7-like RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140
    Illegal BsaI site found at 1204
    Illegal BsaI site found at 2074
    Illegal BsaI.rc site found at 92
    Illegal BsaI.rc site found at 584
    Illegal BsaI.rc site found at 1512
    Illegal BsaI.rc site found at 2522
    Illegal SapI site found at 2503
    Illegal SapI.rc site found at 1698


Design Notes

The sequences were codon optimized.


Source

The Pseudomonas phage Pf-10 RNAP and T7-like promoter are taken from a Pseudomonas phage Pf-10 gene bank. The rest of the sequences are from the iGEM Parts Registry.

References