Composite

Part:BBa_K5250023

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


pT7-like + T7-like RNAP

T7-like RNAP originating from a Pseudomonas phage (BBa_K5250002) is expressed after activation of the T7-like promoter (BBa_K5250003). The promoter is activated by T7-like RNA polymerase (BBa_K5250002). This transcriptional unit is part of the plasmids we are using to test the Feedback loop in our construct.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140
    Illegal BsaI site found at 1204
    Illegal BsaI site found at 2074
    Illegal BsaI.rc site found at 92
    Illegal BsaI.rc site found at 584
    Illegal BsaI.rc site found at 1512
    Illegal BsaI.rc site found at 2522
    Illegal SapI site found at 2503
    Illegal SapI.rc site found at 1698


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