Composite

Part:BBa_K5250022:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


pT7-like + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 725
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 586
    Illegal AgeI site found at 698
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences are codon optimized.


Source

The pT7-like promoter sequence was taken from a Pseudomonas phage gene bank. The rest of the sequences are taken from the iGEM Parts Registry.

References