Composite

Part:BBa_K5250019:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


constitutive promoter + sgRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences are codon optimized.


Source

The sgRNA sequence was designed by the UZurich team members with the help of our lab instructor. The rest of the sequences are taken from the iGEM Parts Registry.

References