Composite

Part:BBa_K5250019

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


constitutive promoter + sgRNA

In this transcription unit we express the sgRNA needed to knock the PDE down via CRISPRi constitutively. In the strain we used for this experiment, a Pseudomonas species IsoF strain, there is a dCas9 encoded chromosomally, which allows the CRISPRi system to knock the gene down. This is one of the biofilm overexpression modules we are testing in our project.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None