Composite

Part:BBa_K5250017:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Prha + DGC WspR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 171
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The parts have been codon optimized.


Source

The source of the WspR gene sequence is this genebank: https://www.ncbi.nlm.nih.gov/gene/878337/ NCBI Reference Sequence: NC_002516.2. The rest of the basic parts in this composite part are taken from the iGEM Part Registry.

References