Composite

Part:BBa_K5250017

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Prha + DGC WspR

In this transcriptional unit the wild type DGC of Pseudomonas aeruginosa is expressed after a rhamnose promoter (BBa_K914003).


Usage and Biology

In our project this is one of the biofilm overexpression modules we are testing. DGC, or diguanylate cyclase, produces the second messenger cyclic di-GMP which is involved in the biofilm production of bacteria. When DGC is overexpressed, this results in the induced formation of biofilm.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1533
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 171
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None