Composite

Part:BBa_K5250016

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Prha + PisoF DGC R240A

A mutated version of the Pseudomonas species PisoF_00565 DGC (BBa_K5250005) is expressed after a rhamnose promoter (BBa_K914003).


Usage and Biology

In our project this is one of the biofilm overexpression modules we are testing. The mutation PisoF_00565 R240A is supposed to inhibit the negative feedback mechanism c-di-GMP has on the DGC activity. We want to increase the level of c-di-GMP, a second messenger involved in biofilm production, and thereby boost biofilm production.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 278
    Illegal BamHI site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 558
    Illegal PstI site found at 627
    Illegal PstI site found at 845
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 989
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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