Composite

Part:BBa_K5250012

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Pxut + GFP

This is a transcription unit activated by the XutR-xylose complex that binds the Pxut promoter. GFP is transcribed.


Usage and Biology

In our project we used this plasmid as part of the testing plasmids for our sensing module. Through the expression of GFP and fluorescence measurements we can determine the activity of the promoter, which will help us test our final construct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 822
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 210
    Illegal AgeI site found at 671
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None