Composite

Part:BBa_K5250011

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


constitutive promoter + XutR

Constitutive promoter expressing XutR

Usage and Biology

This is plasmid that is supposed to help us test the sensing module of our construct. XutR binds to the promoter Pxut in the presence of xylose and triggers expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal BamHI site found at 1236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 669
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None