Coding

Part:BBa_K5250007:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


DGC WspR R242A + Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 760
    Illegal PstI site found at 202
    Illegal PstI site found at 427
    Illegal PstI site found at 499
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 760
    Illegal PstI site found at 202
    Illegal PstI site found at 427
    Illegal PstI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 760
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 760
    Illegal PstI site found at 202
    Illegal PstI site found at 427
    Illegal PstI site found at 499
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 760
    Illegal PstI site found at 202
    Illegal PstI site found at 427
    Illegal PstI site found at 499
    Illegal NgoMIV site found at 124
    Illegal NgoMIV site found at 1026
    Illegal NgoMIV site found at 1032
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been codon optimized. There is a His-tag added for protein purification.

Source

The source of this sequence is the following paper: https://doi.org/10.1371/journal.pbio.0060067

References