Coding

Part:BBa_K5250000

Designed by: Ekaterina Tocheva, Seraina Mueller, Vanessa Doerr   Group: iGEM24_UZurich   (2024-09-28)


XutR

XutR is a protein from the metabolism of Pseudomonas fluorescens which forms a complex with xylose. This complex binds to an operon sequence within the Pxut promoter and activates transcription [1].


Usage and Biology

We used the xylose inducible XutR-Pxut as the sensing module in our construct. It activates our entire genetic circuit and is directly linked to the antitoxin component of our kill switch. It is part of various Level 2 testing plasmids assembled with the Golden Gate system.


References


[1] Callaghan, J. D., Stella, N. A., Lehner, K. M., Treat, B. R., Brothers, K. M., St Leger, A. J., & Shanks, R. M. Q. (2020). Xylose-Inducible promoter tools for pseudomonas species And their use in implicating a role for the type II secretion system protein XCPQ in the inhibition of corneal epithelial wound closure. Applied and Environmental Microbiology, 86(14). https://doi.org/10.1128/aem.00250-20


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 622
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None