Composite

Part:BBa_K5248055:Design

Designed by: Peixin Cai   Group: iGEM24_SZPU-China   (2024-10-02)


pACYCDuet-1-mCherry-mchI（6his)-EGFP-mchB(strep)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 143
    Illegal PstI site found at 369
    Illegal PstI site found at 378
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 143
    Illegal NheI site found at 589
    Illegal PstI site found at 369
    Illegal PstI site found at 378
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 143
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 143
    Illegal PstI site found at 369
    Illegal PstI site found at 378
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 143
    Illegal PstI site found at 369
    Illegal PstI site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References