Composite

Part:BBa_K5246051

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-26)


H.Baltica hfsF-hfsC-hfsI Part of polysaccharide export apparatus

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4218
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2984
    Illegal AgeI site found at 2442
    Illegal AgeI site found at 3783
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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