Composite

Part:BBa_K5246048

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-26)


H.Baltica hfsJ-hfsL-hfsK Part of the polysaccharide tetrad assembly system

Introduction

Vilnius-Lithuania iGEM 2024 project Synhesion aspires to create biodegradable and environmentally friendly adhesives. We were inspired by bacteria, which naturally produce adhesives made from polysaccharides. Two bacteria from aquatic environments - C. crescentus and H. baltica - harness 12 protein synthesis pathways to produce sugars, anchoring them to the surfaces. We aimed to transfer the polysaccharide synthesis pathway to industrially used E. coli bacteria to produce adhesives. Our team concomitantly focused on creating a novel E. coli strain for more efficient production of adhesives.

used in Vilnius-Lithuania iGEM 2024 project "Synhesion" https://2024.igem.wiki/vilnius-lithuania/.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 274
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 708
    Illegal BglII site found at 2877
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 274
    Illegal NgoMIV site found at 1029
    Illegal AgeI site found at 2927
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None