Cell

Part:BBa_K5246040

Designed by: Edgaras Zaboras   Group: iGEM24_Vilnius-Lithuania   (2024-09-23)


HMS147(DE3) ΔWecB

Introduction

Strain description

Usage and Biology

WecB is a UDP-N-acetylglucosamine 2-epimerase that catalyzes the first step in UDP-ManNAcUA(UDP-ManNAcUA) biosynthesis. UDP-ManNAcUA is one of the building blocks for the cell surface polysaccharide enterobacterial common antigen (ECA). WecB synthesizes one of the essential sugars for ECA, and deleting the wecA gene impairs the ECA synthesis pathway in E.coli, therefore lipopolysaccharides containing it are not produced. UDP-ManNAcUA is hypothetically one of the monosaccharides in polysaccharides produced by C. crescentus. We wanted to impair the synthesis of UDP-ManNAcUA to learn about the composition of our polysaccharide. HMS174(DE3) strain is an alternative expression strain to BL21(DE3). Retains the lon protease, which can degrade heterologous proteins. More efficient at expressing non-prokaryotic proteins with rare codons due to its different codon usage bias.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

genotypeF- recA1 hsdR(rK12- mK12+) (DE3) (Rif R)(KanR) ΔwecB

Experimental characterization

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Categories
Parameters
genotypeF- recA1 hsdR(rK12- mK12+) (DE3) (Rif R)(KanR) ΔwecB