Part:BBa_K5246039
Rosetta(DE3)pLysS ΔWecB
Introduction
Strain description
Usage and Biology
WecB is a UDP-N-acetylglucosamine 2-epimerase that catalyzes the first step in UDP-ManNAcUA(UDP-ManNAcUA) biosynthesis. UDP-ManNAcUA is one of the building blocks for the cell surface polysaccharide enterobacterial common antigen (ECA). WecB synthesizes one of the essential sugars for ECA, and deleting the wecA gene impairs the ECA synthesis pathway in E.coli, therefore lipopolysaccharides containing it are not produced. UDP-ManNAcUA is hypothetically one of the monosaccharides in polysaccharides produced by C. crescentus. We wanted to impair the synthesis of UDP-ManNAcUA to learn about the composition of our polysaccharide. Rosetta(DE3)pLysS strain is designed to overcome the limitations of E. coli's codon usage by providing additional tRNAs for certain codons found in eukaryotic proteins. This allows for better expression of these proteins. In Rosetta(DE3)pLysS, the rare tRNA genes and the T7 lysozyme gene are on the same plasmid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
genotype | F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE (CamR)(KanR) ΔwecB |
Experimental characterization
genotype | F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysSRARE (CamR)(KanR) ΔwecB |