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Part:BBa_K524201:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST   (2012-08-19)

Modified ccdB for plasmid assembly on standard vectors


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 682
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 670
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 120


Design Notes

The AvrII site and NheI site were encouraged to exclude from standard biobricks. The addition of two sites but not one is the result considering that either site might be unavoidable in some parts. The arrangement that the AvrII site precedes the NheI site is plainly arbitrary.


Source

Modified P1010 was constructed from BBa_P1010 (ccdB cell death gene) on pSB1C3 by PCR amplification.

References

All information provided was referenced from the BioBrick page of BBa_P1010: https://parts.igem.org/Part:BBa_P1010