Coding

Part:BBa_K5242009

Designed by: Jianting Gong   Group: iGEM24_Tsinghua-M   (2024-09-24)


xylRNLS

xyRNLS, a deterrent protein from B. licheniformis, represses the downstream promoter pxyl in the absence of xylose, and the repressive process is attenuated in the presence of xylose. The role of xyRNAS is illustrated in Figure 1.


Figure 1.The role of xyRNAS is illustrated in Figure 1.

Referances

Chen, Y. et al. Genetic circuit design automation for yeast. Nature Microbiology 5, 1349-1360 (2020).

Teo, W. S. & Chang, M. W. Bacterial XylRs and synthetic promoters function as genetically encoded xylose biosensors in Saccharomyces cerevisiae. Biotechnol J 10, 315-322 (2015). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
  • 1000
    COMPATIBLE WITH RFC[1000]


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