Reporter

Part:BBa_K524006:Design

Designed by: HO Yuan Heng Trevor   Group: iGEM11_HKUST-Hong_Kong   (2011-10-04)

RBS + split sfGFP1-10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 31


Design Notes

A stop codon was added to the end of the CDS to terminate translation of sfGFP1-10. RBS was introduced into primers for cloning.

Source

Constructed from 2011 iGEM DNA Repository Plates and Boxes, Spring Distribution, BBa_I746908

References

Stéphanie Cabantous, Thomas C Terwilliger & Geoffrey S Waldo.(2004).Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.Nature Biotechnology 23, 102 - 107

Stéphanie Cabantous & Geoffrey S Waldo.(2006).In vivo and in vitro protein solubility assays using split GFP.Nature Methods - 3, 845 - 854

Jun Zhou, Jian Lin, Cuihong Zhou, Xiaoyan Deng and Bin Xia.(2011).An improved bimolecular fluorescence complementation tool based on superfolder green fluorescent protein.Acta Biochim Biophys Sin 43 (3): 239-244.