Plasmid

Part:BBa_K5240000

Designed by: Jason Gao   Group: iGEM24_DNHS-SanDiego-CA   (2024-09-21)


Example biosensor with promoter gadB

Example theoretical biosensor using promoter gadB, which could be used to detect butanoyl-homoserine lactone, a signaling molecule used in quorum sensing. It has a resistance marker to protect against carbenicillin and uses the reporter sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3116
    Illegal SpeI site found at 1629
    Illegal PstI site found at 2568
    Illegal PstI site found at 3488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3116
    Illegal SpeI site found at 1629
    Illegal PstI site found at 2568
    Illegal PstI site found at 3488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3116
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3116
    Illegal SpeI site found at 1629
    Illegal PstI site found at 2568
    Illegal PstI site found at 3488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3116
    Illegal SpeI site found at 1629
    Illegal PstI site found at 2568
    Illegal PstI site found at 3488
    Illegal AgeI site found at 2828
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1864
    Illegal BsaI site found at 4965


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Categories
Parameters
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