Plasmid
Part:BBa_K5240000
Designed by: Jason Gao Group: iGEM24_DNHS-SanDiego-CA (2024-09-21)
Example biosensor with promoter gadB
Example theoretical biosensor using promoter gadB, which could be used to detect butanoyl-homoserine lactone, a signaling molecule used in quorum sensing. It has a resistance marker to protect against carbenicillin and uses the reporter sfGFP.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3116
Illegal SpeI site found at 1629
Illegal PstI site found at 2568
Illegal PstI site found at 3488 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3116
Illegal SpeI site found at 1629
Illegal PstI site found at 2568
Illegal PstI site found at 3488 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3116
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3116
Illegal SpeI site found at 1629
Illegal PstI site found at 2568
Illegal PstI site found at 3488 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3116
Illegal SpeI site found at 1629
Illegal PstI site found at 2568
Illegal PstI site found at 3488
Illegal AgeI site found at 2828 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1864
Illegal BsaI site found at 4965
[edit]
Categories
Parameters
None |