Part:BBa_K5238012
The PsppA promoter can increase the expression of target genes in Lactobacillus plantarum
The PsppA promoter is derived from the sakacin P gene cluster and plays a role in driving gene expression. The antimicrobial peptides produced by Lactobacillus plantarum are often regulated through a quorum sensing mechanism based on secreted peptide pheromones. In the expression vector we constructed, the PsppA promoter, along with other regulatory elements, is used to control the expression of the target gene (such as LySKB317). In relevant literature, researchers found that exchanging different promoters, such as the PsppA promoter and PorfX promoter, has an impact on the production of GusA and PepN. In experimental studies, it was found that the PsppA promoter can enhance the production of GusA in two host strains. Therefore, we selected the PsppA promoter in Lactobacillus plantarum to participate in the expression of the target gene in the vector, in order to facilitate the downstream target gene LySKB317 and achieve the function of a suicide switch.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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