Part:BBa_K5238006

Inducing the expression of SrpR repressor under hypoxic conditions

This composite part include a Hypoxia-inducible promoter,a RBS,a coding of SrpR repressor that inhibits transcription of the SrpR-dependent promoter and a terminator.Under low oxygen conditions, it can induce the production of downstream inhibitory proteins, thereby inhibiting endotoxins and promoting the survival of Lactobacillus plantarum under low oxygen conditions.

The promoter PfdhF: fdhF promoter, a hypoxia inducible promoter Hypoxia-inducible promoter. Under hypoxia environment it has a much higher efficiency of starting transcription than under normal 20% oxygen pressure.Characterization of PfdhF, the hypoxia-inducible promoter Yupeng He from [http://2010.igem.org/Team:SJTU-BioX-Shanghai iGEM2010_SJTU-BioX-Shanghai] built and characterized the hypoxia-inducible promoter, fdhF promoter (PfdfF, Part: BBa_K387003). In test experiment, the activity of PfdhF is higher in hypoxia than in normal oxygen pressure (20%). At the beginning, both the hypoxia group and control (20% oxygen partial pressure, i.e. in air) group were incubated with 200 microlitre suspension of bacteria with the testing part (PfdhF followed by firefly luciferase). Result showed that this part worked as we expected: under hypoxia, the efficiency of this promoter was much higher than that under the condition of 20% oxygen at the same OD600.

RBS:we choose BBa_B0034 Designed by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: Antiquity (2003-01-31)

and the coding is to produce SrpR repressor from Pseudomonas putida. It can induce the production of downstream inhibitory proteins, thereby inhibiting endotoxins and promoting the survival of Lactobacillus plantarum under low oxygen conditions

Sequence and Features

Assembly Compatibility:

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  Illegal AgeI site found at 615
  
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  COMPATIBLE WITH RFC[1000]