Coding

Part:BBa_K5236009:Design

Designed by: Hening Guo, Yangzihan Chu   Group: iGEM24_BASIS-China   (2024-09-30)


BhrPETase W229F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 226
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For expressing the sequence, signal peptides and lac operator are suggested to consider when designing a pathway.


Source

This basic part encodes for a mutated BhrPETase W229F and is derived from Escherichia coli.

References