Coding

Part:BBa_K5236007:Design

Designed by: Allen Yang   Group: iGEM24_BASIS-China   (2024-09-30)


IsPETase W159HF229Y


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 864
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 864
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For expressing the sequence, signal peptides and lac operator are suggested to consider when designing a pathway.


Source

Mutation site predicted by AI

References