Regulatory

Part:BBa_K5233043:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-10-01)


SL2, spliced leader 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 100


Design Notes

SL2 should be placed between cds that need to be separated, and the sequence is designed for protein expression in C. elegans

Source

It comes from Caenorhabditis elegans (strain: Bristol N2)

References