Coding

Part:BBa_K5233030

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-10-01)


tphR-PtphC-EGFP

This composite part is a biosensor system that can detect the degradation of PET. It has a promoter that will start the expression of eGFP along with the presence of TPA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 604
    Illegal BamHI site found at 772
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 865
    Illegal NgoMIV site found at 252
    Illegal AgeI site found at 379
    Illegal AgeI site found at 618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703
    Illegal SapI.rc site found at 913


[edit]
Categories
Parameters
None