Coding

Part:BBa_K5233012

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-30)


MHETase(Amcut), a kind of tannase family protein that can degrade MHET

MHETase is a kind of tannase family protein that can degrade MHET. We use it to degrade MHET, the intermediate products, to the TPA. Signal peptide Amcut is used here to make the engineering bacteria secrete the MHETase outside the cell


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1553
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1553
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1553
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1553
    Illegal AgeI site found at 109
    Illegal AgeI site found at 526
    Illegal AgeI site found at 1176
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None