Coding

Part:BBa_K5233009:Design

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-30)


tphR, A kind of regulatory protein that can form complex with TPA which will activate PtphC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 604
    Illegal BamHI site found at 772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 252
    Illegal AgeI site found at 379
    Illegal AgeI site found at 618
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


Design Notes

tphR regulates the activation of PtphC by forming a complex with TPA

Source

It comes from Comamonas thiooxidans strain S23.

References

Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.