Coding

Part:BBa_K5233008

Designed by: Tianshen TANG   Group: iGEM24_HUST-UEVE-UPSaclay   (2024-09-28)


tphC, a kind of substrate-binding protein (SBP) belonging to the tripartite tricarboxylate transport

tphC is a kind of substrate-binding protein (SBP) belonging to the tripartite tricarboxylate transporters (TTT) class of transporters. It binds TPA and enables tpiA, tpiB to transport TPA into the cell. We use it to binding TPA to transport it into bacteria cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
    Illegal BamHI site found at 976
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 808
    Illegal NgoMIV site found at 735
    Illegal AgeI site found at 70
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None