RNA

Part:BBa_K5232015

Designed by: Prof. Tomoji Mashimo (Extra Adviser)   Group: iGEM24_UTokyo   (2024-09-28)

Usage and Biology

Activated by recognizing the elongation products from ds-template (BBa_K5232003) and primer3 (BBa_K5232007). It is responsible for cleavage of ssDNA in the reporter molecule by its collateral activity. Here we register crRNA.

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Figure 1.

Cas3-Cascade/crRNA complex recognizes a double-stranded PAM sequence and a single-stranded spacer sequence for activation [1]. When ds Amp and Cas3 system were connected, no increase in NC fluorescence intensity was observed, and the graph showed a slope dependent on target concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Yoshimi, K., Takeshita, K., Kodera, N., Shibumura, S., Yamauchi, Y., Omatsu, M., Umeda, K., Kunihiro, Y., Yamamoto, M., & Mashimo, T. (2022). Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3. Nature Communications, 13(4917), 1-11. <https://doi.org/10.1038/s41467-022-32618-0


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