Part:BBa_K5232011

Usage and Biology

The 3' end side has a sequence complementary to hsa-miR-375-5p, and the 5' end side is the template strand for primer0 ^[1].

Figure 1.

Figure 2.

The larger this ratio, the more clearly the target concentration can be distinguished. For Biomarker 2, when the condition was 10-5, that is, when the hybridize length between template and target was 10 bp, and the hybridize length between template and helper was 5 bp, the ratio was the largest.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Qing, Z., Feng, C.,Feng, X., Yongxi, Z., & Chunhai, F. (2014). Target-Triggered Three-Way Junction Structure and Polymerase/Nicking Enzyme Synergetic Isothermal Quadratic DNA Machine for Highly Specific, One-Step, and Rapid MicroRNA Detection at Attomolar Level. Anal. Chem. 2014, 86, 16, 8098-8105. https://doi-org.utokyo.idm.oclc.org/10.1021/ac501038r