Primer

Part:BBa_K5232006

Designed by: Dr. Ken Komiya (Extra Adviser)   Group: iGEM24_UTokyo   (2024-09-28)

Usage and Biology

The amplification product of the first step of the multistep-SDA reaction.

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Figure 1.
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Figure 2.

By using a template with TAMRA modification at the 3' end, amplification was confirmed within 200 min of the observation. For step 123, amplification was confirmed when the target concentration was 100 pM or higher. Amplification of NCs was not confirmed during the observation time.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Komiya, K., Noda, C. & Yamamura, M. (2024). Characterization of Cascaded DNA Generation Reaction for Amplifying DNA Signal.New Gener. Comput. 42, 237-252. https://doi.org/10.1007/s00354-024-00249-2


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Categories
Parameters
None