Coding

Part:BBa_K523024

Designed by: Chris French, Eugene Fletcher, Sylvia Ispasanie   Group: iGEM11_Edinburgh   (2011-10-28)

cex + cenA fusion (cxnA)

Cex exoglucanase from Cellulomonas fimi fused to CenA endoglucanase from the same species. The part contains, in order:

  • The GH10 exoglucanase domain
  • A (possibly inactivated) cellulose binding domain
  • The GH6 endoglucanase domain

Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part BBa_K523025.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 524
    Illegal NotI site found at 2222
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2135
    Illegal XhoI site found at 1633
    Illegal XhoI site found at 1882
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 530
    Illegal NgoMIV site found at 1032
    Illegal NgoMIV site found at 1413
    Illegal NgoMIV site found at 2338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577
    Illegal SapI.rc site found at 660


[edit]
Categories
Parameters
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