Composite

Part:BBa_K523007

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-07-29)

Plac + LacZ + malS (dubious version)

E. coli periplasmic alpha-amylase gene malS under control of lac promoter. LacZ is also present for blue/white screening.

This contains an artificial RBS, and no stop codon... furthermore the 2 extra G bases at the end of the malS sequence (see BBa_K523003) mean there's not even a stop codon provided by the BioBrick suffix, since it's now in a different frame.

This part might therefore not be of any use to anyone, even ourselves...


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1830
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 625
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 966
    Illegal AgeI site found at 2131
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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