Coding
Part:BBa_K523003:Design
Designed by: Mun Ching Lee, Sylvia Ispasanie Group: iGEM11_Edinburgh (2011-07-29)
malS for BioSandwich
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1204
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 340
Illegal AgeI site found at 1505 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.
The part was created from a lab E. coli strain using primers:
- ACT AGATCT atg aaa ctc gcc gcc tgt ttt c
- Forward; adds BglII site; starts at start codon.
- ACT ACTAGT ACC ctg ttg ccc tgc cca gac g
- Reverse; adds SpeI site; RFC23 and BioSandwich compliant; no stop codons, adds a glycine.
Source
The source E. coli genome sequence can be seen using GenBank U00096.2