Part:BBa_K5226084

Mmp1-phaP

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

In order to produce multiple classes of high value-added bioproducts while absorbing assimilated CDE, based on the current research of related literature, we iGEM24-SCUT-China-A chose to introduce the synthesis pathway of PHA particle-associated protein.

Usage and Biology

The phaP gene produces a protein that binds to the surface of hydrophobic polyhydroxyalkanoate (PHA) particles and plays an important role in regulating PHA synthesis and possibly protein folding. To test the ability of Halomonas TD to produce phaP protein, we chose to use the inducible promoter Mmp1. In this experiment given, we added 50 mg/L of IPTG to the shake flask fermentation system for induced fermentations

Experimental characterisation

growth conditions

shake flask studies

experimental design

Data Processing and Analysis

The fermentation broth was centrifuged to obtain the supernatant and precipitate after cell disruption, and they were subjected to SDS-PAGE, and the following results were obtained after shooting: from left to right, the protein results obtained by fermentation under two carbon sources, sodium acetate and glucose, were phaP, TD80 control, SOD, Amalase, Meta and sodium acetate and glucose. It can be seen that compared with the control group, the TD80 after the introduction of protein synthesis pathway genes can produce phaP and SOD proteins, and the protein produced with glucose as the carbon source is more than that with sodium acetate as the carbon source.

References

[1] Engineering Halomonas spp. as A Low-Cost Production Host for Production of Bio-surfactant Protein PhaP